NETosis Colorimetric Assay Kit (E-BC-K884-M)
Add to cart
For research use only.
| Detection Principle |
NETosis is an inflammatory cell death mode of neutrophils. Activated neutrophils capture and kill pathogens by releasing Neutrophil extracellular traps (NETs), which are composed of deaggregated chromatin and intracellular granule proteins. The formation of NETs is accompanied by the death of neutrophils. This new type of death is different from apoptosis and necrosis and is called NETosis. There are many inducers of NETosis, such as pathogens (bacteria, fungi, viruses, etc.), activated platelets, chemokines (interleukins, granulocyte colony-stimulating factor, transforming growth factor β, etc.), artificial ingredients and calcium ionophore, etc. These inducers can promote the key proteins of NETosis, Neutrophil Elastase (NE), Myeloperoxidase (MPO) and Peptidearginine Deaminase IV (PAD4) transcription and translation. This method evaluated the degree of NETosis by detecting the enzyme activity of NE. |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (360-400 nm, optimum wavelength: 380 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Basal culture medium, DMSO |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.31 U/L |
| Detection Range | 0.31-18.33 U/L |
| Precision | inter-assay CV: 4.8-7.4% | intra-assay CV: 2.1-3.8% |
| Sample Volume | 20 μL(cell homogenate) |
| Assay Time | 7 h 20 min |
| Cat.No. | Product Name | Clone No. |
|---|
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
