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For research use only.

Verified Samples Verified Samples in WB: HepG2, Rat thymus, Rat heart
Verified Samples in IHC: Mouse liver, Rat spleen
Dilution WB 1:500-1:1000,  IHC 1:100-1:300
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC
Clonality Polyclonal
Immunogen Recombinant Human Programmed cell death protein 4 protein
Abbre PDCD4
Synonyms Death up-regulated gene protein,  Dug,  H731,  MGC33046,  MGC33047,  Ma3,  Neoplastic transformation inhibitor,  Neoplastic transformation inhibitor protein,  Nuclear antigen H731,  Nuclear antigen H731 like,  Nuclear antigen H731 like protein,  Nuclear antigen H731-like,  PDCD
Swissprot
Calculated MW 50,51 kDa
Observed MW 56 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm, Nucleus.
Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Research Areas Cancer,  Cell Biology,  Epigenetics and Nuclear Signaling
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation,progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.
Other Clones

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Unconjugated

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