For research use only.
| Verified Samples |
Verified Samples in WB: NIH/3T3, RAW264.7 Verified Samples in IHC: Human cervical cancer, Human thyroid cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human PDIA4 |
| Abbre | PDIA4 |
| Synonyms | Calcium binding protein intestinal related, EPR70, EPR72, ER protein 70, ER protein 72, ERP 70, ERp 72, ERp-72, ERp70, ERp72, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, PDIA 4, PDIA4, PDIR, Pdia4, Protein disulfide isomerase A4 |
| Swissprot | |
| Calculated MW | 73 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Concentration | 0.9 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | PDIA4(Protein disulfide-isomerase A4) is also named as ERP70,ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal,KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif,CGHC. The full length protein has a signal peptide with 20 amino acid. |
Other Clones
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