Phospho-P38 (Thr180/Tyr182) Polyclonal Antibody (E-AB-21027)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse lung, Mouse liver, Rat kidney, HepG2, HepG2, 3T3, 3T3 Verified Samples in IHC: Human colon Verified Samples in IF: Mouse liver |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human p38 around the phosphorylation site of Thr180/Tyr182 |
| Abbre | p38 (phospho Thr180/Tyr182) |
| Synonyms | CSAID Binding Protein 1, CSAID binding protein, CSAID-binding protein, CSBP, CSBP 1, CSBP 2, CSBP1, CSBP2, CSPB1, Csaids binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, EXIP, MAP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14 |
| Swissprot | |
| Calculated MW | 41 kDa |
| Observed MW |
38 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Tissue Specificity | Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Immunology, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A,p38MAPK,CSBP1,RK,p38,EXIP,Mxi2,CSBP2,PRKM14,PRKM15,CSPB1,p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing. |
Other Clones
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Unconjugated
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