Phytase Activity Colorimetric Assay Kit (E-BC-K1211-M)
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For research use only.
| Detection Principle |
Phytase is the general term for a class of enzymes that catalyze the hydrolysis of phytic acid and its salts into inositol and phosphate. It belongs to the category of phosphate monoester hydrolases. Phytase has a special spatial structure and can sequentially separate the phosphorus in phytic acid molecules, converting phytic acid (salt) into inositol and inorganic phosphorus, while releasing other nutrients bound to phytic acid. The detection principle of this kit: Phytase can decompose the substrate to produce inorganic phosphorus. Under acidic conditions, inorganic phosphorus reacts with the molybdate ammonium chromogenic agent to produce blue molybdenum blue substance. The content of inorganic phosphorus can be detected at a wavelength of 660 nm to calculate the activity of phytase. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (650-670 nm, optimum wavelength: 660 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.001 U/L |
| Detection Range | 0.001-0.24 U/L |
| Precision | inter-assay CV: 4.8%-7.2% | intra-assay CV: 3.6%-4.5% |
| Sample Volume | 100 μL(tissue homogenate/cell homogenate) |
| Assay Time | 1 h 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Spinach tissue homogenate | 1 |
| 10% Bean Sprouts tissue homogenate | 1 |
| 10% Broccoli tissue homogenate | 1 |
| 10% Pumpkin tissue homogenate | 1 |
| 10% Chive tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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