PODXL Polyclonal Antibody (D-AB-10420L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, Raji, HepG2 |
| Dilution | WB 1:250-1:500 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human PODXL protein expressed by E.coli |
| Abbre | PODXL |
| Synonyms | Gp200, PC, PCLP, PCLP 1, PCLP1, PODXL, Podocalyxin, Podocalyxin like protein 1, RCC marker, Renal Cell Carcinoma marker, TRA 1 81, TRA-1-60, podocalyxin like, GCTM 2 antigen, TRA 1 60 |
| Swissprot | |
| Calculated MW | 55 kDa |
| Observed MW |
160 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Cardiovascular, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Podocalyxin,also known as podocalyxin-like protein 1 (PODXL or PCLP1),is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus,but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition,podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ～55.5 kDa that undergoes a post‐translational glycosylation,the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns |
Other Clones
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Other Formats
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Unconjugated
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