For research use only.
| Verified Samples |
Verified Samples in WB: Mouse liver, Mouse skeletal muscle Verified Samples in IHC: Human colorectal cancer, Human thyroid cancer |
| Dilution | WB 1:500-1:2000, IHC 1:25-1:50 |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human POMC |
| Abbre | POMC |
| Synonyms | ACTH, Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, CLIP, Corticotropin, Corticotropin lipotropin, Corticotropin-like intermediary p, alpha-MSH, alphaMSH, beta-MSH |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 0.96 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Metabolism, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes,including regulating energy metabolism,immune reactions,and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP,γ-MSH,ACTH,α-MSH,CLIP,Lipotropin β,Lipotropin γ,β-MSH,β endorphin and Met-enkephalin. |
Other Clones
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