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Porcine TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit (E-EL-P3016)

All Size Price Qty
96T $ 682.00
48T $ 546.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
Add to cart

For research use only.

Product Summary
Sensitivity 1.88 ng/mL
Detection Range 3.13-200 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Porcine
Specificity This kit recognizes TFPI in samples.No significant cross-reactivity or interference between TFPI and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TFPI . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TFPI , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TFPI . You can calculate the concentration of TFPI in the samples by comparing the OD of the samples to the standard curve.
This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization.
Uniport ID F1RV85
Research Area Cardiovascular
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