PPIB Monoclonal Antibody (E-AB-22170)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, Jurkat, 293T, Rat liver, 3T3, HepG2 Verified Samples in IHC: Human hepatocarcinoma, Human pancreas carcinoma |
| Dilution | WB 1:1000-2000, IHC 1:100-200 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide |
| Abbre | Cyclophilin B |
| Synonyms | AA408962, AA553318, AI844835, CYP S1, CYP-S1, CYPB, Cphn 2, Cphn2, CyP 20b, Cyclophilin B, Cyclophilin like protein, EC 5.2.1.8, MGC14109, MGC2224, OI9, Peptidyl prolyl cis trans isomerase B precursor, Peptidyl-prolyl cis-trans isome, peptidyl prolyl cis trans isomerase B |
| Swissprot | |
| Observed MW |
21 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Immunology, Signal Transduction |
| Clone No. | 1B3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. |
Other Clones
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Other Formats
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Unconjugated
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