Purified Anti-Mouse CD27 Antibody[LG.3A10], Functional Grade (AN003220)
For research use only.
Conjugation: None (AF/LE)
APC Elab Fluor®488 Elab Fluor®647 FITC None (AF/LE) PE Unconjugated Show More
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes cell |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), FA Reported in the literature |
| Isotype | Armenian Hamster IgG1, κ |
| Host | Armenian Hamster |
| Reactivity | Mouse |
| Applications | FCM, FA |
| Clonality | Monoclonal |
| Immunogen | Recombinant Mouse CD27 protein |
| Isotype Control | Purified Armenian Hamster IgG Isotype Control[PIP], Functional Grade |
| Abbre | CD27 |
| Synonyms | CD27 antigen, CD27L receptor, S152, T-cell activation antigen CD27, Tp55, Tumor necrosis factor receptor superfamily member 7, Tnfrsf, Tnfrsf7, Cd27, T14, S152, TNFRSF7 |
| Swissprot | |
| Cellular Localization | Cell membrane |
| Tissue Specificity | In thymus and spleen, but not in non-lymphoid tissues. Expressed on myeloid progenitor cells. |
| Concentration | ≥ 1 mg/mL |
| Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Purification Method | >98%, Protein A/G purified |
| Clone | LG.3A10 |
| Conjugation | None (AF/LE) |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice bag |
| background | CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
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