Purified Anti-Mouse CD27 Antibody[LG.3A10], Functional Grade (AN003220)
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For research use only.
| Alternate Names | CD27 antigen, CD27L receptor, S152, T-cell activation antigen CD27, Tp55, Tumor necrosis factor receptor superfamily member 7, Tnfrsf, Tnfrsf7, Cd27, T14, S152, TNFRSF7 |
| Clone | |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes cell |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);FA Reported in the literature |
| Background | CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse CD27 protein |
| Abbre | CD27 |
| Swissprot | |
| Host | Armenian Hamster |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Armenian Hamster IgG1, κ |
| Isotype Control | Purified Armenian Hamster IgG Isotype Control[PIP], Functional Grade |
| Applications |
FCM;FA
|
| Cellular Localization |
Cell membrane
|
| Tissue Specificity |
In thymus and spleen, but not in non-lymphoid tissues. Expressed on myeloid progenitor cells.
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
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