Purified Anti-Mouse IL-2 Antibody[JES6-5H4], Functional Grade (E-AB-F12010)
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For research use only.
| Alternate Names | IL-2, IL2, Interleukin-2, T-cell growth factor, TCGF |
| Clone | |
| Leadtime | Inquire |
| Verified Samples | Verified Samples in FCM: HEK293T cells transfected with pcDNA3.1 plasmid encoding Mouse IL-2 gene |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Neut Reported in the literature;Stim Reported in the literature |
| Background | Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell prolifeRation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse IL-2 protein |
| Abbre | IL-2 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG2b, κ |
| Isotype Control | Purified Rat IgG2b, κ Isotype Control[LTF-2], Functional Grade |
| Applications |
ICFCM;Neut;Stim
|
| Research Areas | Immunology |
| Cellular Localization |
Secreted
|
| Tissue Specificity |
Immune cells including dendritic cells
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Shivani Srivastava, et al. J Exp Med. 2014 May 5;211(5):961-74. Ana Villegas-Mendez, et al. Infect Immun. 2015 Oct 12;84(1):34-46. |
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