Recombinant 4E-BP1/EIF4EBP1 Monoclonal Antibody (AN300048P)
For research use only.
| Verified Samples | Verified Samples in WB: 293T, K562 |
| Dilution | WB 1:1000-1:5000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human 4E-BP1 / EIF4EBP1 protein |
| Abbre | EIF4EBP1 |
| Synonyms | 4EBP, eIF4E binding protein, eukaryotic translation initiation factor 4E-binding protein, 4E-BP, EIF4EBP, EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, eukaryotic translation initiation factor 4E-binding protein 1, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1, 4EBP1, 4E-BP1, BP-1, eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I |
| Swissprot | |
| Calculated MW | 13 kDa |
| Observed MW |
20 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Cytosol. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Metabolism |
| Clone No. | 2C4 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
Other Clones
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Unconjugated
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