Recombinant AACT Monoclonal Antibody (AN301913L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human plasma Verified Samples in IHC: Human placenta |
| Dilution | WB 1:5000-1:10000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human AACT fragment |
| Abbre | AACT |
| Synonyms | MGC, SERPINA, Serpin A, GIG, SERPINA3, AACT, ACT, GIG24, GIG25, Alpha-1-Antichymotrypsin, Cell Growth-Inhibiting Gene 24/25 Protein, MGC88254, Serpin A3, Alpha-1-antichymotrypsin His-Pro-less, Antichymotrypsin, antitrypsin) member 3, Cell growth inhibiting gene 24/25 protein, Growth inhibiting protein 24, Growth inhibiting protein 25, Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, Serine (or cysteine) proteinase inhibitor clade A member 3, Serine proteinase inhibitor clade A member 3, Serpin family A member 3, Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 |
| Swissprot | |
| Calculated MW | 48 kDa |
| Observed MW |
55-70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Cardiovascular, Cell Biology |
| Clone No. | A629 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Alpha 1-antichymotrypsin (AACT), also known as Human SerpinA3, is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy. |
Other Clones
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Other Formats
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Unconjugated
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