Recombinant ABCE1 Monoclonal Antibody (AN301424L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Panc-1, Mouse liver Verified Samples in IF: HeLa, NIH-3T3 |
| Dilution | WB 1:500-1:1000, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human ABCE1 fragment |
| Abbre | ABCE1 |
| Synonyms | ABC, ABCE, RNASEL, OK/SW-cl, ABCE1, ABC38, OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I, OK/SW-cl.40, 2' 5' oligoadenylate binding protein, 2'5' oligoadenylate binding protein, 2''-5''-oligoadenylate-binding protein, ABC 38, ABCE 1, ATP binding cassette sub family E (OABP) member 1, ATP binding cassette sub family E member 1, ATP-binding cassette sub-family E member 1, HuHP 68, HuHP68, Ribonuclease 4 inhibitor, Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) inhibitor, Ribonuclease L (2'5' oligoisoadenylate synthetase dependent) inhibitor, Ribonuclease L inhibitor, RNase L inhibitor, RNS 4I |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Mitochondrion |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Immunology |
| Clone No. | A119 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway. Together with PELO and HBS1L, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes. Together with PELO and HBS1L, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway. Plays a role in the regulation of mRNA turnover. Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA. As part of the PINK1-regulated signaling, ubiquitinated by CNOT4 upon mitochondria damage; this modification generates polyubiquitin signals that recruit autophagy receptors to the mitochondrial outer membrane and initiate mitophagy. RNASEL-specific protein inhibitor which antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) to RNASEL. Negative regulator of the anti-viral effect of the interferon-regulated 2-5A/RNASEL pathway. |
Other Clones
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Unconjugated
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