Recombinant Aconitase 1/ACO1 Monoclonal Antibody (AN301871L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse kidney, Rat kidney, Human liver tissue lysate, the Aconitase1/ACO1 Rabbit mAb pre-adsorbed with 3μM of the synthetic peptides, Human liver tissue lysate, the Aconitase 1/ACO1 Rabbit mAb with no peptide blocking. |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Aconitase 1/ACO1 fragment |
| Abbre | Aconitase 1/ACO1 |
| Synonyms | HEL, IRP, ACO, IREB, ACO1, ACONS, HEL60, IREB1, IREBP, IREBP1, IRP1, ACO 1, ACOC, Aconitase, Aconitase 1 soluble, Aconitase1, Aconitate hydratase, Citrate hydro lyase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRE BP 1, IREB 1, IRE-BP 1, Iron regulatory protein 1, Iron responsive element binding protein 1, Iron-responsive element-binding protein 1, IRP 1, OTTHUMP00000045233 |
| Swissprot | |
| Calculated MW | 98 kDa |
| Observed MW |
105 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular, Signal Transduction, Epigenetics and Nuclear Signaling, Metabolism |
| Clone No. | A583 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Aconitase 1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. |
Other Clones
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Unconjugated
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