Recombinant ADA2 Monoclonal Antibody (AN302007L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HUVEC (negative?control),?MOLT-4 Verified Samples in IHC: Human tonsil, Human colon, Human skeletal muscle (negative tissue) |
| Dilution | WB 1:1000, IHC 1:1000-1:2000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | ADA2 |
| Synonyms | ADA, Cat eye syndrome critical region protein, Cat eye syndrome chromosome region candidate, PAN, ADGF, CECR1, IDGFL, SNEDS, VAIHS, Cat eye syndrome chromosome region candidate 1, Cat eye syndrome critical region protein 1, Cat eye syndrome critical region protein 1 precursor, CECR 1, ADA2, ADGF, CECR1, IDGFL, SNEDS, VAIHS |
| Swissprot | |
| Calculated MW | 59 kDa |
| Observed MW |
65 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A727 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | ADA2, also named as TADA2A, is a subunit of the ADA2A-containing (ATAC) histone acetyltransferase complex, which with histone acetyltransferase activity on histones H3 and H4. It is required for the function of some acidic activation domains, which activate transcription from a distant site. It has a role in chromatin remodeling by binding at the linker region between neighbouring unclesomes of dinucleosomes. |
| Cat.No. | Product Name | Clone No. |
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