Recombinant AKR1B1 Monoclonal Antibody (AN300120P)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, Hela, A431 Verified Samples in IF: Hela Verified Samples in IP: Jurkat |
| Dilution | WB 1:500-1:2000, ICC/IF 1:20-1:100, IP 0.2-1 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, ICC/IF, IP |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human AKR1B1 protein |
| Abbre | AKR1B1 |
| Synonyms | ALR, MGC, ALDR, AKR1B, AKR1B1, ADR, ALDR1, ALR2, AR, MGC1804, AKR1B 1, Aldehyde reductase, Aldehyde reductase 1, Aldo keto reductase family 1, aldo-keto reductase family 1, Aldo-keto reductase family 1 member B1, Aldose reductase, aldr 1, Lii5 2 CTCL tumor antigen, Lii5-2 CTCL tumor antigen|aldehyde reductase 1|aldo-keto reductase family 1, Low Km aldose reductase, member B1, member B1 (aldose reductase), member B1|aldose reductase|low Km aldose reductase |
| Swissprot | |
| Calculated MW | 35 kDa |
| Observed MW |
37 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Metabolism |
| Clone No. | 9D9 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 37 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. |
Other Clones
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Unconjugated
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