Recombinant ALDH5A1/SSADH Monoclonal Antibody (AN301828L)
For research use only.
| Verified Samples | Verified Samples in WB: A431, HepG2, Human liver |
| Dilution | WB 1:200-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human ALDH5A1/SSADH fragment |
| Abbre | ALDH5A1/SSADH |
| Synonyms | ALDH5A, SSADH, ALDH5A1, Aldedehyde dehydrogenase 5 family, Aldehyde dehydrogenase 5 family, Aldehyde dehydrogenase 5 family member A1, Aldehyde dehydrogenase 5A1, Aldehyde dehydrogenase family 5 member A1, ALDH5A 1, member A1, mitochondrial, Mitochondrial succinate semialdehyde dehydrogenase, NAD(+) dependent succinic semialdehyde dehydrogenase, NAD(+)-dependent succinic semialdehyde dehydrogenase, SSDH, Succinate semialdehyde dehydrogenase, Succinate-semialdehyde dehydrogenase |
| Swissprot | |
| Calculated MW | 57 kDa |
| Observed MW |
54, 57 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Signal Transduction, Metabolism |
| Clone No. | A540 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | ALDH5A1 catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA, 4-aminobutyrate; gamma-aminobutyrate). GABA, a derivative of the excitatory neurotransmitter glutamate, is the primary inhibitory neurotransmitter in the central nervous system. Following release and interaction with postsynaptic receptors, GABA is resorbed into the presynaptic neurons and is either reused or metabolized. |
Other Clones
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Unconjugated
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