Recombinant alpha Actinin 4 Monoclonal Antibody (AN301433L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, HEK-293, 4T1, Raw264.7 Verified Samples in IHC: Human colon Verified Samples in IF: HeLa, Raw264.7 |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Actinin 4 fragment |
| Abbre | alpha Actinin 4 |
| Synonyms | ACTN, Non-muscle alpha-actinin, ACTININ, ACTN4, ACTININ-4, FSGS, FSGS1, Non-muscle alpha-actinin 4, actinin 4, Actinin alpha 4, actinin4, ACTN 4, Alpha-actinin-4, DKFZp686K23158, F actin cross linking protein, F-actin cross-linking protein, Focal segmental glomerulosclerosis 1, FSGS 1, Non muscle alpha actinin 4 |
| Swissprot | |
| Calculated MW | 100 kDa |
| Observed MW |
110 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell junction, Cytoplasm, Cytoskeleton, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Clone No. | A128 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA. |
Other Clones
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Other Formats
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Unconjugated
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