Recombinant Alpha Skeletal Muscle Actin Monoclonal Antibody (AN301797L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, MCF-7, NIH/3T3, Mouse brain, Rat heart Verified Samples in IHC: Human prostate cancer, Mouse skeletal muscle, Rat skeletal muscle Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:1000-1:5000, IHC 1:200-1:1000, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Alpha Skeletal Muscle fragment |
| Abbre | Alpha Skeletal Muscle Actin |
| Synonyms | NEM1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM2, NEM3, ACTA, ACTA1, a actin, Actin, Actin alpha skeletal muscle, actina, actine, ACTS, aktin, alpha 1, Alpha Actin 1, alpha skeletal muscle, Alpha skeletal muscle Actin, alpha-actin, Alpha-actin-1, nemaline myopathy type 3, skeletal muscle, skeletal muscle 1 |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW |
42 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoskeleton |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags & Cell Markers, Signal Transduction, Cancer, Isotype, Loading Controls |
| Clone No. | A509 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. |
Other Clones
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Unconjugated
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