Recombinant B7-H3/CD276 Monoclonal Antibody (AN301442L)

Name: Recombinant B7-H3/CD276 Monoclonal Antibody
Brand: Elabscience
SKU: AN301442L
Price: 249.0 USD
Availability: InStock

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Recombinant B7-H3/CD276 Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: HeLa, MCF-7, 293T Verified Samples in IHC: Human cervical cancer, Human lung squamous carcinoma, Human tonsil, Mouse spleen, Mouse stomach Verified Samples in IF: HeLa, THP-1 Verified Samples in FCM: THP-1, HeLa Verified Samples in IP: HeLa cells extracts
Dilution	WB 1:500-1:2000, IHC 1:200-1:1000, IF 1:50, FCM 1:50-1:100, IP 1:25-1:100
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Mouse
Applications	WB, IHC, IF, FCM, IP
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human B7-H3/CD276 fragment
Abbre	B7-H3/CD276
Synonyms	B7H, UNQ, PRO, B7-H, B7RP, B7 homolog, B7H34Ig-B7-H, PSEC, 4Ig-B7-H, CD276, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, B7 homolog 3, B7H34Ig-B7-H3, CD276 antigen, CD276 molecule, Costimulatory molecule, PSEC0249, UNQ309, PRO352, B7RP-2, 4Ig-B7-H3, B7H3, B7-H3, CD276 antigen
Swissprot	Q5ZPR3
Calculated MW	57 kDa
Observed MW	110 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Membrane
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Immunology, Cancer, Stem Cells
Clone No.	A137
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.