Recombinant BAP31 Monoclonal Antibody (AN301443L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, 293, 4T1, Rat liver Verified Samples in IHC: Human brain Verified Samples in IF: U-2 OS |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human BAP31 fragment |
| Abbre | BAP31 |
| Synonyms | BAP, BCAP, BAP31, DXS1357E, BCAP31, 6C6 AG, 6C6 AG tumor associated antigen, 6C6AG, 6C6AG tumor associated antigen, 6C6-AG tumor-associated antigen, Accessory protein BAP 31, Accessory protein BAP31, B cell receptor associated protein 31, BA31, BAP 31, BCAP 31, B-cell receptor-associated protein 31, BCR associated protein Bap 31, BCR associated protein Bap31, BCR-associated protein 31, CDM, CDM protein, MS950, p28, p28 Bap31, Protein CDM, RP23-329M9.5 |
| Swissprot | |
| Calculated MW | 28 kDa |
| Observed MW |
28 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum, Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology |
| Clone No. | A138 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Functions as a chaperone protein. Is one of the most abundant endoplasmic reticulum (ER) proteins. Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). Also serves as a cargo receptor for the export of transmembrane proteins. May be involved in CASP8-mediated apoptosis. |
Other Clones
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Other Formats
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Unconjugated
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