Recombinant Bcr Monoclonal Antibody (AN301879L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, HEK-293, PC-12 Verified Samples in IHC: Human cerebrum, Human kidney, Human placenta |
| Dilution | WB 1:500-1:1000, IHC 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Bcr fragment |
| Abbre | Bcr |
| Synonyms | Renal carcinoma antigen NY-REN, BCR, ALL, BCR1, CML, D22S11, D22S662, PHL, Breakpoint cluster region, Renal carcinoma antigen NY-REN-26, bcr, BCR1, Breakpoint cluster region, CML, D22S11, D22S662, PHL, Renal carcinoma antigen NY-REN-26 |
| Swissprot | |
| Calculated MW | 160 kDa |
| Observed MW |
160 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Synapse, dendritic spine, axon |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer |
| Clone No. | A591 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Break point cluster region protein (Bcr) is encoded by the Bcr gene. The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine / threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia. |
Other Clones
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Other Formats
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Unconjugated
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