Recombinant bFGF/FGF2 Monoclonal Antibody (AN300097P)

Name: Recombinant bFGF/FGF2 Monoclonal Antibody
Brand: Elabscience
SKU: AN300097P
Price: 380.0 USD
Availability: InStock

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Recombinant bFGF/FGF2 Monoclonal Antibody - 1

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Verified Samples	Verified Samples in WB:HeLa
Dilution	WB: 1:1000；ICC/IF: 1:200-1:500；FC: 1:200-1:500；IP: 1:20-1:50
Isotype	IgG
Host	Rabbit
Reactivity	Human, Mouse
Applications	WB, ICC/IF, FC, IP
Clonality	Recombinant;Monoclonal
Immunogen	A synthetic peptide corresponding to the N-terminus of the Human bFGF.
Abbre	FGF2
Synonyms	HBGF, FGF, BFGF, FGF-2, FGFB, HBGF-2, Basic FGF, FGF2, Basic Fibroblast Growth Factor, B-FGF, FGF-β, Fibroblast Growth Factor 2, heparin-binding growth factor 2, prostatropin, BFGF, B-FGF, FGF-2, FGFB, fibroblast growth factor 2, HBGF-2, heparin-binding growth factor 2, prostatropin, basic, Basic fibroblast growth factor bFGF, FGF 2, FGF B, FGF2 basic, Fibroblast growth factor, Fibroblast growth factor 2 (basic), HBGF 2, HBGF2, HBGH 2, HBGH2, Heparin binding growth factor 2 precursor, heparin-binding growth factor 2, FGF-β
Swissprot	P09038
Calculated MW	31 kDa
Observed MW	19 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Secreted, Nucleus.
Tissue Specificity	Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Concentration	1 mg/mL
Buffer	0.2 μm filtered solution in PBS
Purification Method	Protein A
Research Areas	Cardiovascular, Signal Transduction, Neuroscience, Stem Cells, Cancer, Developmental Biology
Clone	A1211
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]