Recombinant BOB-1/OBF-1 Monoclonal Antibody (AN301720L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Ramos, Daudi Verified Samples in IF: EL4, Ramos Verified Samples in IP: Daudi cells extracts |
| Dilution | WB 1:1000-1:2000, IF 1:50, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human BOB-1 / OBF-1 fragment |
| Abbre | BOB-1/OBF-1 |
| Synonyms | BOB, POU2AF, OBF, POU2AF1, BOB1, OBF-1, OBF1, OCAB, POU2AF1, BOB1, OBF-1, OBF1, OCAB |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
35 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Epigenetics and Nuclear Signaling |
| Clone No. | A428 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity. |
Other Clones
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Other Formats
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Unconjugated
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