Recombinant BRG1 Monoclonal Antibody (AN300890L)
For research use only.
| Verified Samples |
Verified Samples in WB: C6 Verified Samples in IHC: Human breast carcinoma |
| Dilution | IHC 1:200-1:1000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Applications | IHC, WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human BRG1 protein |
| Abbre | BRG1 |
| Synonyms | BRG, SNF, CSS, BAF, MRD, SWI, RTPS, SNF2L, SMARCA, BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b, SMARCA4, SNF2B, actin dependent regulator of chromatin, ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF 190, Brahma protein like 1, BRG1 associated factor 190A, BRG1 protein, BRG1-associated factor 190A, BRM/SWI2 related gene 1, Global transcription activator homologous sequence, global transcription activator snf2l4, Homeotic gene regulator, matrix associated, member 4, Mitotic growth and transcription activator, Nuclear protein GRB1, Protein brahma homolog 1, Protein BRG1, Protein BRG-1, SMARC A4, SMCA4, SNF2 beta, SNF2 like 4, SNF2-beta, subfamily a, Sucrose nonfermenting like 4, SWI/SNF related, SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, Transcription activator BRG1 |
| Swissprot | |
| Calculated MW | 185 kDa |
| Observed MW |
220 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Epigenetics and Nuclear Signaling, Stem Cells, Cancer |
| Clone No. | 7C2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member ofThe SWI/SNF family of proteins and is similar toThe brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by alteringThe chromatin structure around those genes.The encoded protein is part ofThe large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulateThe expression ofThe tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. |
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