Recombinant Caldesmon/CDM Monoclonal Antibody (AN301452L)
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For research use only.
| Verified Samples |
Verified Samples in WB: C2C12 Verified Samples in IHC: Human colon, Mouse colon, Rat colon Verified Samples in IF: HeLa |
| Dilution | WB 1:500-1:1000, IHC 1:200-1:1000, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Caldesmon/CDM fragment |
| Abbre | Caldesmon/CDM |
| Synonyms | NAG, MGC, CALD1, CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, caldesmon, CALD 1, Caldesmon 1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, Caldesmon1, H CAD, L CAD, MGC21352, CAD, CAD, CALD 1, CALD1, CALD1, Caldesmon 1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, Caldesmon, Caldesmon1, CDM, H CAD, HCAD, L CAD, LCAD, MGC21352, NAG22 |
| Swissprot | |
| Calculated MW | 93 kDa |
| Observed MW |
75 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoskeleton |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cardiovascular |
| Clone No. | A147 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. |
Other Clones
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Unconjugated
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