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Recombinant Caspase 3 Monoclonal Antibody (E-AB-81444)

All Size Price Qty
100μL $ 260.00
50μL $ 160.00
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For research use only.

Verified Samples Verified Samples in WB: K562, Hela
Verified Samples in IHC: Human tonsil
Dilution WB 1:500-1:1000,  IHC 1:50-1:100
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse
Applications WB,  IHC-P
Clonality Rabbit Monoclonal
Immunogen A synthetic peptide of human Caspase 3
Synonyms A830040C14Rik,  Apopain,  CASP-3,  CASP3,  CC3,  CPP-32,  CPP32,  CPP32B,  Casp3a,  Caspase 3,  Caspase-3 subunit p12,  Cysteine p,  apoptosis-related cysteine peptidase,  apoptosis-related cysteine protease,  apoptosis-related cysteine protease a
Swissprot
Calculated MW 32 kDa
Observed MW 32 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm.
Concentration 300 μg/mL
Buffer 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification Method Affinity Purified
Research Areas Cancer,  Cell Biology,  Metabolism,  Neuroscience
Clone No. R06-1B2
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
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Unconjugated

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