Recombinant CCR6 Monoclonal Antibody (AN301459L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Raji, HL-60 Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:25000-1:50000, IHC 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CCR6 fragment |
| Abbre | CCR6 |
| Synonyms | DCR, DRY, GPR, CKRL, STRL, CKR-L, CMKBR, GPRCY, CC-CKR, C-C CKR, CCR, CCR6, BN-1, C-C CKR-6, CC-CKR-6, CCR-6, CD196, CKR-L3, CKRL3, CMKBR6, DCR2, DRY6, GPR29, GPRCY4, STRL22, BN 1, BN1, C C chemokine receptor type 6, C C CKR 6, CC chemokine receptor type 6, C-C chemokine receptor type 6, CC CKR 6, CC R6, CCCKR6, CCR 6, CD 196, CD196 antigen, Chemokine (C C motif) receptor 6, Chemokine (C C) receptor 6, Chemokine (CC motif) receptor 6, Chemokine (CC) receptor 6, Chemokine receptor 6, Chemokine receptor like 3, Chemokine receptor-like 3, CKR 6, CKR L3, CKR6, CMKBR 6, DCR 2, DRY 6, G protein coupled receptor 29, GPR 29, GPR CY4, GPRCY 4, GPR-CY4, G-protein coupled receptor 29, LARC receptor, Seven transmembrane receptor lymphocyte 22, STRL 22 |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW |
42 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Signal Transduction, Cardiovascular |
| Clone No. | A154 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins. Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity. Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma. |
Other Clones
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Unconjugated
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