Recombinant CD16a Monoclonal Antibody (AN301467L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat Verified Samples in IHC: Human liver, Human spleen, Human cervical cancer, Human liver cancer |
| Dilution | WB 1:500-1:1000, IHC 1:200-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD16a fragment |
| Abbre | CD16a |
| Synonyms | FCR, IMD, FCG, Fcgr, IGFR, IgG Fc Receptor III, CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3, IMD20, FCGR3A, CD16a Antigen, Fc-Gamma RIII, Fc-gamma RIIIa, Fc-Gamma RIII-Alpha, IgG Fc Receptor III-2, Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A, Fc&, gamma-RIIIA-A, CD16a, CD 16, CD 16a, CD16B, CD16b antigen, Fc fragment of IgG, Fc fragment of IgG low affinity IIIa receptor, Fc fragment of IgG low affinity IIIa receptor (CD16), Fc fragment of IgG receptor IIIa, Fc gamma R3, Fc gamma receptor III 2 (CD 16), Fc gamma receptor III A, Fc gamma receptor IIIA, Fc gamma receptor IIIb (CD 16), Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIII beta, Fc gamma RIIIa, Fc gamma RIIIb, Fc of IgG, FCG 3, FCG3A, Fc-gamma receptor III2 (CD 16), Fc-gamma receptor III2 (CD16), Fc-gamma receptor IIIb (CD16), FCgammaRIIIA, FCGR 3, FCGR 3A, FCGR3A protein, FCGRIII-2, FcR 10, FcR10, IGFR 3, IgG Fc receptor III 1, IgG Fc receptor III 2, immunoglobulin G Fc receptor III, immunoglobulin G Fc receptor III-2, low affinity III, low affinity IIIa, Low affinity IIIa receptor, low affinity IIIb, Low affinity immunoglobulin gamma Fc region receptor III A, Low affinity immunoglobulin gamma Fc region receptor IIIB, neutrophil-specific antigen NA, receptor (CD16), receptor (CD16a), receptor (CD16b), receptor for, receptor for (CD16) |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
50-70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Stem Cells |
| Clone No. | A162 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. |
Other Clones
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Unconjugated
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