Recombinant CD20 Monoclonal Antibody (AN301470L)

Name: Recombinant CD20 Monoclonal Antibody
Brand: Elabscience
SKU: AN301470L
Price: 249.0 USD
Availability: InStock

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Recombinant CD20 Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: Raji Verified Samples in IHC: Human spleen, Mouse spleen, Rat spleen Verified Samples in FCM: Ramos Verified Samples in IP: Raji cells extracts
Dilution	WB 1:1000-1:5000, IHC 1:200-1:1000, FCM 1:50-1:100, IP 1:50-1:100
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC, FCM, IP
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human CD20 fragment
Abbre	CD20
Synonyms	LEU, CVID, B-lymphocyte antigen CD, Leukocyte surface antigen Leu, B-lymphocyte surface antigen B, Membrane-spanning 4-domains subfamily A member, MS4A1, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, Bp35, Ly-44, APY, ATOPY, B lymphocyte antigen CD20, B Lymphocyte Cell Surface Antigen B1, B-lymphocyte cell-surface antigen B1, Bp 35, CD 20, CD20 antigen, CD20 receptor, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU 16, LEU16, leukocyte surface antigen Leu 16, Ly44, Membrane spanning 4 domains A1, Membrane spanning 4 domains subfamily A member 2, MGC3969
Swissprot	P11836
Calculated MW	33 kDa
Observed MW	35 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cytoplasm and plasma membrane
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Immunology, Stem Cells, Cancer
Clone No.	A165
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.