Recombinant CD239/BCAM Monoclonal Antibody (AN301706L)
For research use only.
| Verified Samples |
Verified Samples in WB: A431 Verified Samples in IHC: Human kidney Verified Samples in FCM: HeLa |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:100, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD239/BCAM fragment |
| Abbre | CD239/BCAM |
| Synonyms | MSK, BCAM, AU, CD239, LU, MSK19, Auberger B antigen, Basal cell adhesion molecule, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein, Rh30CE, Antigen identified by monoclonal F8, B CAM cell surface glycoprotein, B cell adhesion molecule, Basal cell adhesion molecule (Lu and Au blood groups), Basal cell adhesion molecule (Lutheran blood group), Basal cell adhesion molecule Lu and Au blood groups, Basal cell adhesion molecule Lutheran blood group, CD239 antigen, Glycoprotein 95kDa, Lutheran, Lutheran blood group (Auberger b antigen included), Lutheran blood group Auberger b antigen included |
| Swissprot | |
| Calculated MW | 67 kDa |
| Observed MW |
85,88 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular, Immunology |
| Clone No. | A414 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. |
Other Clones
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Other Formats
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Unconjugated
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