Recombinant CD36 Monoclonal Antibody (AN300923L)

For research use only.
Verified Samples | Verified Samples in WB: Mouse spleen |
Dilution | WB 1:1000-1:5000 |
Isotype | IgG,κ |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Applications | WB |
Clonality | Monoclonal;Recombinant |
Immunogen | Recombinant Human CD36 protein |
Abbre | CD36 |
Synonyms | PAS, CHDS, Leukocyte differentiation antigen CD, BDPLT, SCARB, FATCHDS, BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3, CD36, CD36 Molecule(Thrombospondin Receptor), FATCHDS7, Fatty acid translocase, Glycoprotein IIIb, GP88, GPIIIB, Platelet collagen receptor, Thrombospondin receptor, PAS-4, PAS IV, Fatty acid translocase (FAT), Glycoprotein IIIb (GPIIIB), Leukocyte differentiation antigen CD36, gpIIIb, Adipocyte membrane protein, CD36 antigen, CD36 antigen (collagen type I receptor, CD36 molecule, CD36 molecule (thrombospondin receptor), CD36 Molecule(Thrombospondin Receptor), Cluster determinant 36, Collagen receptor, Fatty acid transport protein, GP IIIb, MGC108510, MGC91634, PAS 4 protein, platelet, Platelet glycoprotein 4, Platelet glycoprotein IV, Scavenger receptor class B member 3, thrombospondin receptor) |
Swissprot | |
Calculated MW | 53 kDa |
Observed MW |
90 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Membrane |
Concentration | 0.2 mg/mL |
Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
Purification Method | Protein A |
Research Areas | Cardiovascular, Immunology, Microbiology, Stem Cells, Cancer, Metabolism |
Clone No. | 9A8 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. |
Other Clones
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Unconjugated
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