Recombinant CD39 Monoclonal Antibody (AN301981L)
For research use only.
| Verified Samples | Verified Samples in WB: Human placenta, Mouse lung |
| Dilution | WB 1:1000-1:2000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant fragment |
| Abbre | CD39 |
| Synonyms | SPG, Ecto-ATP diphosphohydrolase, Ectonucleoside triphosphate diphosphohydrolase, ENTPD, ENTPD1, ATPDase, CD39, NTPDase-1, SPG64, CD 39, NTPDase1, Ecto-apyrase, Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Lymphoid cell activation antigen, NTPDase-1, CD39 antigen, DKFZp686D194, DKFZp686I093, Ecto apyrase, Ecto ATP diphosphohydrolase, Ecto-ATPase 1, Ecto-ATPDase 1, ENTP1, ENTPD 1, FLJ40921, FLJ40959 |
| Swissprot | |
| Calculated MW | 58 kDa |
| Observed MW |
78 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology |
| Clone No. | A701 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CD39,also known as ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), is a multi-pass membrane ectoenzyme that metabolizes adenosine-5'-triphosphate (ATP) to regulate purinergic signaling. Purinergic signaling by extracellular ATP and its metabolites regulate many biological processes, including vascular tone, digestion, neuronal function, and inflammation in both normal and diseased states. CD39 is expressed in endothelial cells in the vasculature to regulate local platelet purinergic signaling via metabolism of ATP to adenosine. Accordingly, CD39 regulates platelet activation aggregation and contributes to the antithrombotic properties of endothelial cells. ATP and its metabolites also finely modulate the activity of T cells and macrophages. Immunomodulation is regulated, in part, by the availability of extracellular ATP and adenosine, suggesting that CD39 may play an immunosuppressive role in the tumor microenvironment. |
Other Clones
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