Recombinant CD41 Monoclonal Antibody (AN301478L)
For research use only.
| Verified Samples | Verified Samples in WB: Human platelet |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD41 fragment |
| Abbre | CD41 |
| Synonyms | HPA, PPP1R, ITGA2B, BDPLT16, BDPLT2, CD41, CD41B, GP2B, GPIIb, GT, GTA, HPA3, PPP1R93, GPalpha Iib, Integrin alpha-Iib, Platelet membrane glycoprotein Iib, ITGAB, &, alpha-IIb integrin-n, BDPLT16, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, alpha subunit, antigen CD41, antigen CD41), form 2, Integrin, Integrin alpha 2b, Integrin alpha IIb, Integrin alpha-IIb light chain, integrin αⅡbβ3, ITA2B, platelet fibrinogen receptor, platelet glycoprotein IIb of IIb/IIIa complex, platelet specific antigen BAK |
| Swissprot | |
| Calculated MW | 113 kDa |
| Observed MW |
128 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular, Immunology, Signal Transduction, Stem Cells |
| Clone No. | A173 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Integrin α-IIb/β-3 (ITGA2B) is a cell surface adhesion molecule that is the receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. In activated platelets, ITGA2B can be cleaved by proteolytic enzymes to generate disulfide bond-connected heavy and light chains, and form fibrinogen receptors together with glycoprotein IIIb, causing platelet interactions and rapid platelet aggregation, thereby Block the surface of ruptured endothelial cells. The corresponding ITGA2B gene mutation can cause platelet weakness. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
