Recombinant CDK1/Cdc2 Monoclonal Antibody (AN301969L)
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For research use only.
| Verified Samples |
Verified Samples in WB: A20, BRL, Rat brain, COS-7, HeLa, A431 Verified Samples in IHC: Human tonsil Verified Samples in IF: HeLa Verified Samples in FCM: A20, HeLa |
| Dilution | WB 1:1000, IHC 1:50-1:100, IF 1:50-1:100, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse, African green monkey |
| Applications | WB, IHC, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CDK1/Cdc2 fragment |
| Abbre | CDK1/Cdc2 |
| Synonyms | CDC, CDK, CDKN, cyclin-dependent kinase, P34CDC, CDK1, CDC2, CDC28A, P34CDC2, cyclin-dependent kinase 1, CDKN1, CDC2, CDC28A, cyclin-dependent kinase 1, P34CDC2, cyclin dependent kinase 1874 |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
34 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoskeleton, Cytoplasm, Nucleus, Mitochondrion |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Cancer, Neuroscience, Metabolism |
| Clone No. | A685 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CDC2, also named as CDK1, belongs to the protein kinase superfamily. The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15. Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases. |
Other Clones
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Other Formats
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Unconjugated
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