Recombinant Chromogranin A/CHGA/CGA Monoclonal Antibody (AN300072P)
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For research use only.
| Verified Samples |
Verified Samples in WB: SH-SY5Y in IHC: Rat pancreas, Human pancreas, Human stomach, Mouse pancreas, Mouse pancreas in IF: Mouse pancreas, Mouse pancreas, Rat pancreas |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000, IF 1:200-1:1000, ELISA 1:5000-1:20000, IP 1:50-1:200, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| Clonality | Recombinant;Monoclonal |
| Immunogen | A synthetic peptide corresponding to the center region of the Human Chromogranin A/CHGA/CGA |
| Abbre | CHGA |
| Synonyms | CHGA, CGA, Chromogranin-A, Pituitary secretory protein I, SP-I, Chromogranin A |
| Swissprot | |
| Calculated MW | 51kD |
| Observed MW |
80kD
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, Cytoplasmic vesicle. |
| Tissue Specificity | Detected in cerebrospinal fluid (at protein level). Detected in urine (at protein level). |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Tags and Cell Markers, Signal Transduction, Cancer |
| Clone | A1182 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
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