Recombinant Claudin-1 Monoclonal Antibody (AN301492L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 Verified Samples in IHC: Human cervix, Human colon cancer, Human cervical cancer Verified Samples in IP: HepG2 cells extracts |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Claudin-1 fragment |
| Abbre | Claudin-1 |
| Synonyms | UNQ, PRO, CLD, CLDN1, CLD1, ILVASC, SEMP1, claudin-1, Claudin1, CLD 1, CLDN 1, SEMP 1, Senescence associated epithelial membrane protein, Senescence associated epithelial membrane protein 1, Senescence-associated epithelial membrane protein, UNQ481, PRO944, claudin-1, CLD1, ILVASC, SEMP1 |
| Swissprot | |
| Calculated MW | 22 kDa |
| Observed MW |
18 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction |
| Clone No. | A187 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions. |
Other Clones
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Other Formats
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Unconjugated
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