Recombinant Cleaved PARP1 p85 Monoclonal Antibody (AN301495L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Jurkat, EL4.IL2, PC-12 Verified Samples in IF: HeLa Verified Samples in FCM: HeLa, NIH/3T3 Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:500-1:2000, IF 1:100-1:200, FCM 1:50-1:100, IP 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IF, FCM, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Cleaved PARP1 p85 fragment |
| Abbre | Cleaved PARP1 p85 |
| Synonyms | SCA, ARTD, Poly[ADP-ribose] synthase, Poly ADP ribose polymerase, poly(ADP-ribose) polymerase, Poly [ADP-ribose] polymerase, NAD(+) ADP-ribosyltransferase, ADP-ribosyltransferase diphtheria toxin-like, pADPRT, ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1, PARP1, Cleaved PARP, ADP-ribosyltransferase diphtheria toxin-like 1, APOPAIN, NAD(+) ADP-ribosyltransferase 1, Poly [ADP-ribose] polymerase 1, Poly ADP ribose polymerase 1, poly(ADP-ribose) polymerase 1, Poly[ADP-ribose] synthase 1, SCA1, ADPRT, ADPRT 1, ADPRT1, ARTD1, pADPRT-1, PARP, PARP-1, poly(ADP-ribose) polymerase 1, PPOL |
| Swissprot | |
| Calculated MW | 85kDa |
| Observed MW |
89kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Metabolism, Cancer |
| Clone No. | A190 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. |
Other Clones
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