Recombinant Clusterin beta Chain Monoclonal Antibody (AN301900L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse serum, Rat serum |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Clusterin beta Chain fragment |
| Abbre | Clusterin beta Chain |
| Synonyms | KUB, AAG, Ku70-binding protein, Sulfated glycoprotein, Complement-associated protein SP, Testosterone-repressed prostate message, NA1/NA, SGP, CLU, AAG4, APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, clusterin, CLUS, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40, Ku70-binding protein 1, Sulfated glycoprotein2, Testosterone-repressed prostate message 2, Complement cytolysis inhibitor a chain, Clusterin alpha chain, Complement cytolysis inhibitor b chain, APOJ CLI, Sulfated glycoprotein 2 |
| Swissprot | |
| Calculated MW | 52 kDa |
| Observed MW |
35 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion membrane, Secreted, Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Cardiovascular, Signal Transduction, Cancer, Metabolism |
| Clone No. | A616 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Clusterin (CLU) also named as apolipoprotein J, is a multifunctional glycoprotein that is expressed ubiquitously in most tissues. Clusterin functions as a secreted chaperone protein that interacts with and stabilizes stress-induced proteins to prevent their precipitation. Research studies show that clusterin plays a protective role in Alzheimer’s disease by sequestering amyloid β (1-40) peptides to form long-lived, stable complexes, which prevents amyloid fibril formation.In addition to the secreted protein, several intracellular isoforms are localized to the nucleus, mitochondria, cytoplasm and ER. The subcellular distribution of these multiple isoforms leads to the diversity of clusterin functions. Additional studies report that clusterin is involved in membrane recycling, cell adhesion, cell proliferation, apoptosis, and tumor survival. |
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Unconjugated
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