Recombinant EGR2 Monoclonal Antibody (AN302056L)
For research use only.
| Verified Samples | Verified Samples in WB: Human glioma,?Mouse brain, Rat brain |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | EGR2 |
| Synonyms | EGR, KROX, EGR2, AT591, CMT1D, CMT4E, KROX20, DKFZp686J1957, Drosophila, E3 SUMO-protein ligase EGR2, early growth response 2, Early growth response protein 2, EGR-2, FLJ14547, homolog (early growth response-2), KROX 20 Drosophila homolog, Krox 20 homolog Drosophila, KROX-20, Krox20 protein, Zinc finger protein Krox-20 |
| Swissprot | |
| Calculated MW | 50 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling, Neuroscience |
| Clone No. | A776 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | EGR2 is also named as KROX20, belongs to the EGR C2H2-type zinc-finger protein family. As a zinc finger transcription factor, it is observed in both the somata and dendrites of central neurons. EGR2 plays an important role in the transient formation of hindbrain developmental compartments or rhombomeres and is also an important factor in peripheral myelination, maintenance of synaptic plasticity and long-term potentiation. EGR2 expression is induced downstream of TCR signaling in NKT precursors and EGR2 is directly connected with the key molecular checkpoints defining NKT lineage commitment and stage progression, suggesting that EGR2 not only induces the early lineage-defining transcription factor PLZF, but also controls the downstream expression of the IL-2Rβ chain. EGR2 is regulated by both soluble and membrane-bound neuregulins and its concentration is partially modulated by calcium-dependent events. EGR2/Krox20 is also required for induction of Pmp22, and is nuclear-localized. |
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Unconjugated
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