Recombinant EIF2S1 Monoclonal Antibody (AN301512L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, HepG2, MCF-7, Raw264.7, C6 Verified Samples in IHC: Human breast |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human EIF2S1 fragment |
| Abbre | EIF2S1 |
| Synonyms | EIF2S, EIF, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1, EIF 2, EIF 2 alpha, EIF 2A, EIF 2alpha, EIF2 alpha, eIF-2-alpha, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, Eukaryotic translation initiation factor 2 subunit alpha, IF2A |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
36 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A211 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The eukaryotic translation initiation factor 2-α subunit (EIF2S1) is the first regulatory step that catalyzes the initiation of protein synthesis and promotes the binding of the initial tRNA to the 40S ribosomal subunit. As an important translation initiation factor, the eukaryotic initiation factor 2-α subunit is regulated by a variety of signal molecules, that is, it functions by regulating the phosphorylation and acetylation status of EIF2S1. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate EIF2S1 to regulate the transcription and translation of cell-related genes and enhance Cell protection mechanism. |
Other Clones
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Unconjugated
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