Recombinant FAK Monoclonal Antibody (E-AB-81468)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, C6, 3T3, Hela Verified Samples in IHC: Human colon cancer |
| Dilution | WB 1:1000-1:2000, IHC 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human FAK |
| Abbre | FAK |
| Synonyms | FADK, FADK 1, FAK related non kinase polypeptide, FAK1, FRNK, Focal adhesion Kinase, Focal adhesion kinase 1, Focal adhesion kinase isoform FAK Del33, Focal adhesion kinase related nonkinase, PPP1R71, Protein phosphatase 1 regulatory subunit 7, p125FAK, pp125FAK |
| Swissprot | |
| Calculated MW | 119 kDa |
| Observed MW |
125 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell junction>focal adhesion. Cell membrane. Constituent of focal adhesions. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cardiovascular, Signal Transduction |
| Clone No. | R06-6B6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | FAK (Focal adhesion kinase 1) is also named as FAK1,FADK,pp125FAK,FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion,migration,proliferation and survival in response to extracellular signals . It also acts as a pivotal signal 'integrator',controlling and coordinating cellular responses that include cell migration,survival,proliferation and,epithelial tissue repair after DNA damage . This protein has 6 isoforms produced by alternative promoter usage and alternative splicing. |
Other Clones
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Unconjugated
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