Recombinant FGL1 Monoclonal Antibody (AN301921L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, Human liver Verified Samples in IHC: Human hepatocellular carcinoma, Human liver, Human cerebrum(Negative tissue) |
| Dilution | WB 1:1000, IHC 1:50-1:200 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human FGL1 fragment |
| Abbre | FGL1 |
| Synonyms | FGL, Fibrinogen-like protein, Liver fibrinogen-related protein, LFIRE, HP-041, Hepassocin, HPS, HFREP-1, LFIRE-1, HFREP1, Fibrinogen-like protein 1, Liver fibrinogen-related protein 1, FGL1, Fgl1, Hepassocin, HFREP1, HFREP-1, FGL 1, Fibrinogen like 1, Fibrinogen like protein 1, Fibrinogen related protein 1, Hepatocellular carcinoma related sequence, Hepatocyte derived fibrinogen related protein 1, Hepatocyte-derived fibrinogen-related protein 1, HFREP 1, HP 041, HP041, LFIRE 1, LFIRE1, Liver fibrinogen related protein 1, MFIRE 1, MGC108569, MGC12455, MGC37822, OTTHUMP00000122468 |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
34 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular |
| Clone No. | A637 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Fibrinogen-like protein 1 (FGL1), also known as hepassocin, is a secreted protein mainly expressed in liver and its expression is further enhanced following acute liver injury. FGL1 null mice have multiple metabolic abnormalities and are more prone to develop hepatocellular carcinoma under certain experimental conditions, suggesting FGL1 has diverse functions in vivo. More recently, FGL1 was found to be highly expressed in human cancer cells and function as an inhibitory ligand for LAG3. Blockade of the FGL1-LAG3 interaction stimulates tumor immunity in mouse models. In addition, a high plasma FGL1 level in human cancer patients is associated with a poor prognosis and resistance to anti-PD-1/PD-L1 immunotherapy. |
Other Clones
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