Recombinant GAPDH Monoclonal Antibody (AN004430L)
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For research use only.
| Verified Samples |
Verified Samples in WB: A431, Caco-2, Jurkat, K562, HeLa, PC-3, Ramos, TF-1, U20-S, HL-60, HT-29, Raji, HepG2, Mouse heart, Mouse liver, Mouse uterus, Mouse lung, Mouse kidney, Mouse brain, Mouse stomach, C6, PC-12, Rat heart, Rat liver, Rat spleen, Rat brain, Rat stomach, Rat testis, Rat thymus, Rat skeletal muscle Verified Samples in IHC: Human colon cancer, Human appendix, Human breast cancer, Human lung cancer, Rat ovary, Mouse ovary Verified Samples in IF: U-2 OS cells, NIH/3T3 cells, C6 |
| Dilution | WB 1:20000-1:100000, IHC 1:2000-1:4000, IF 1:2000-1:4000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF |
| Clonality | Recombination |
| Immunogen | Recombinant protein within Mouse GAPDH |
| Abbre | GAPDH |
| Synonyms | BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
36 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Isotype, Loading Controls, Signal Transduction, Cancer, Metabolism |
| Clone No. | 20B8 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | GAPDH is an enzyme of 36kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR. |
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Unconjugated
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