Recombinant GLUT2 Monoclonal Antibody (AN301695L)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, HUVEC, NIH-3T3, RAW264.7, C6, PC-12 Verified Samples in FCM: HepG2 |
| Dilution | WB 1:500-1:1000, FCM 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human GLUT2 fragment |
| Abbre | GLUT2 |
| Synonyms | GLUT, SLC2A, SLC2A2, GLUT2, facilitated glucose transporter member 2, Glucose transporter, Glucose Transporter 2, Glucose Transporter GLUT2, Glucose transporter type 2, Glucose transporter type 2 liver, GLUT-2, GTR2, GTT2, liver, liver/islet, Solute carrier family 2, Solute carrier family 2 (facilitated glucose transporter) member 2, Solute carrier family 2 facilitated glucose transporter member 2, solute carrier family 2 member 2 |
| Swissprot | |
| Calculated MW | 57 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Stem Cells, Developmental Biology, Cancer, Metabolism |
| Clone No. | A403 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | GLUT2 is a facilitative hexose transporter that mediates the transport of glucose and fructose, which likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. GLUT2 may participate with the Na+/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney and is also able to mediate the transport of dehydroascorbate. |
Other Clones
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Other Formats
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Unconjugated
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