Recombinant Glypican 3 Monoclonal Antibody (AN301534L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 Verified Samples in IF: HepG2 Verified Samples in FCM: HepG2 Verified Samples in IP: HepG2 cells extracts |
| Dilution | WB 1:500-1:2000, IF 1:50, FCM 1:50-1:100, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IF, FCM, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Glypican 3 fragment |
| Abbre | Glypican 3 |
| Synonyms | MXR, GPC, UNQ, PRO, CDw, ADAM, Protein FOAP, Secreted glypican, Glypican proteoglycan, Sialic acid-binding Ig-like lectin, C20orf, OCI, GPC3, DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, glypican-3, CDw329, Glypican 3, Glypican proteoglycan 3, Glypican-3 [Precursor], GTR2 2, Heparan sulphate proteoglycan, Intestinal protein OCI 5, Intestinal protein OCI-5, OCI 5, Protein FOAP-9, Secreted glypican-3, Sialic acid-binding Ig-like lectin 9, SIGLEC9, Siglec-9, ADAM33, C20orf153, UNQ873, PRO1891, OCI5 |
| Swissprot | |
| Calculated MW | 66 kDa |
| Observed MW |
66 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Stem Cells, Signal Transduction, Cancer, Developmental Biology |
| Clone No. | A233 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. |
Other Clones
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Unconjugated
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