Recombinant GOLGA2/GM130 Monoclonal Antibody (AN302050L)
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For research use only.
| Verified Samples |
Verified Samples in WB: K562, HeLa, HepG2, Neuro-2a Verified Samples in IHC: Human colon, Human breast cancer, Mouse spleen, Rat stomach |
| Dilution | WB 1:1000, IHC 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | GOLGA2/GM130 |
| Synonyms | GOLGA, golgin A, GOLGA2, GM130, golgin A2, 130 kDa cis Golgi matrix protein, 130 kDa cis-Golgi matrix protein, Cis golgi matrix protein GM130, Gm130 autoantigen, GOGA2, GOLGA 2, Golgi autoantigen, Golgi autoantigen golgin subfamily a 2, Golgi matrix protein GM130, Golgin 95, Golgin subfamily a 2, Golgin subfamily A member 2, Golgin-95, MGC20672, SY11 protein |
| Swissprot | |
| Calculated MW | 113 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Golgi apparatus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags & Cell Markers, Signal Transduction |
| Clone No. | A770 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus,and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Required for normal protein transport from the endoplasmicreticulum to the Golgi apparatus and the cell membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at thecis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgidisassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPIvesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly andcentrosome organization. |
Other Clones
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Unconjugated
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