Recombinant GOT1 Monoclonal Antibody (AN301537L)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse heart, Rat heart Verified Samples in IHC: Human cerebrum, Rat kidney |
| Dilution | WB 1:1000-1:2000, IHC 1:100-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human GOT1 fragment |
| Abbre | GOT1 |
| Synonyms | GIG, GOT, ASTQTL, GOT1, AST1, ASTQTL1, GIG18, cAspAT, cCAT, AAT, ASAT, AspAT, serum glutamic oxaloacetic transaminase, SGOT, AST, cytoplasmic, Glut, Aspartate aminotransferase, AATC, Aspartate aminotransferase 1, Aspartate aminotransferase cytoplasmic, Aspartate aminotransferase cytosolic, cysteine aminotransferase, cysteine transaminase, cytoplasmic (cCAT), ec 2.6.1.1, GIG 18, Glutamate oxaloacetate transaminase 1, Glutamate oxaloacetate transaminase soluble, Glutamic oxaloacetic transaminase 1 soluble, GOT 1, Growth inhibiting protein 18, Transaminase A |
| Swissprot | |
| Calculated MW | 46 kDa |
| Observed MW |
46 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Clone No. | A236 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. |
Other Clones
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Unconjugated
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